Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells
نویسندگان
چکیده
منابع مشابه
Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells
OBJECTIVES The diverse clinical applications for human mesenchymal stem cells (hMSCs) in cellular therapy and regenerative medicine warrant increased focus on developing adequate culture supplements devoid of animal-derived products. In the present study, we have investigated the feasibility of umbilical cord blood-platelet lysate (UCB-PL) as a standard substitute for fetal bovine serum (FBS) a...
متن کاملPathogen reduction through additive-free short-wave UV light irradiation retains the optimal efficacy of human platelet lysate for the expansion of human bone marrow mesenchymal stem cells
BACKGROUND We recently developed and characterized a standardized and clinical grade human Platelet Lysate (hPL) that constitutes an advantageous substitute for fetal bovine serum (FBS) for human mesenchymal stem cell (hMSC) expansion required in cell therapy procedures, avoiding xenogenic risks (virological and immunological) and ethical issues. Because of the progressive use of pathogen-reduc...
متن کاملEXPANSION OF HUMAN CORD BLOOD PRIMITIVE PROGENITORS IN SERUM-FREE MEDIA USING HUMAN BONE MARROW MESENCHYMAL STEM CELLS
Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem ...
متن کاملhuman platelet lysate as a xeno free alternative of fetal bovine serum for the in vitro expansion of human mesenchymal stromal cells
background: mesenchymal stromal cells (mscs) are employed in various different clinical settings in order to modulate immune response. human autologous and allogeneic supplements including platelet derivatives such as platelet lysate (pl), platelet-released factors (prf) and serum are assessed in clinical studies to replace fetal bovine serum (fbs). the immunosuppressive activity and multi-pote...
متن کاملDonor Age of Human Platelet Lysate Affects Proliferation and Differentiation of Mesenchymal Stem Cells
The regenerative potential declines upon aging. This might be due to cell-intrinsic changes in stem and progenitor cells or to influences by the microenvironment. Mesenchymal stem cells (MSC) raise high hopes in regenerative medicine. They are usually culture expanded in media with fetal calf serum (FCS) or other serum supplements such as human platelet lysate (HPL). In this study, we have anal...
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ژورنال
عنوان ژورنال: Journal of Translational Medicine
سال: 2014
ISSN: 1479-5876
DOI: 10.1186/1479-5876-12-28